Recombinant Cynomolgus BAFF / TNFSF13B / CD257 Trimer Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Thr141-Leu285 trimer design.
Molecular Weight:
The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 90% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Form:
Lyophilized
Sequence:
Thr141-Leu285
Target:
BAFF/TNFSF13B/CD257
Cynomolgus BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Cynomolgus BAFF Trimer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC50 of 31.6ng/ml determined by ELISA.
The purity of Cynomolgus BAFF Trimer is greater than 90% as determined by SEC-HPLC.
Immobilized Cynomolgus BAFF (Trimer), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BAFFR, hFc Tag with the EC50 of 60.0ng/ml determined by ELISA.
Loaded Cynomolgus/Rhesus macaque BAFFR, hFc Tag on ProA-Biosensor can bind Cynomolgus BAFF (Trimer), His-Avi Tag with an affinity constant of 1.23 nM as determined in BLI assay (Gator Prime).
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