Recombinant Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus.It contains Thr141-Leu285 trimer design.
Molecular Weight:
The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 90% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Form:
Lyophilized
Sequence:
Thr141-Leu285
Target:
BAFF/TNFSF13B/CD257
Biotinylated Cynomolgus BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Biotinylated Cynomolgus BAFF (Trimer), His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA (QC Test).
The purity of Biotinylated Cynomolgus BAFF Trimer is greater than 90% as determined by SEC-HPLC.
Biotinylated Cynomolgus BAFF (Trimer), His Tag captured on CM5 Chip via streptavidin can bind Cynomolgus BCMA, His Tag with an affinity constant of 1.40 µM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Cynomolgus BAFF (Trimer), His-Avi-Flag Tag on Streptavidin-Biosensor, can bind Cynomolgus BCMA, His Tag with an affinity constant of 1.69 µM as determined in BLI assay (Gator).
Loaded Human BAFFR, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus BAFF (Trimer), His-Avi-Flag Tag with an affinity constant of 0.68 nM as determined in BLI assay (Gator Prime).
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