Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 90% as determined by Bis-Tris PAGE
Form:
Lyophilized
Sequence:
Ser7-Ala71
Target:
BAFFR/TNFRSF13C
Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF Trimer, hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
Human BAFFR on Bis-Tris PAGE under reduced conditions. The purity is greater than 90%.
Immobilized Human BAFFR, His Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 6.2 ng/ml determined by ELISA.
Immobilized Human BAFFR, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test).
Loaded Human BAFFR, His Tag on Anti-His-Biosensor can bind Human BAFF (Trimer), monomeric Fc-Flag Tag with an affinity constant of 0.98 nM as determined in BLI assay (Gator Prime).
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