Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE
Form:
Lyophilized
Sequence:
Met1-Thr49
Target:
BCMA/TNFRSF17
Mouse BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse BCMA, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test).
Immobilized Mouse BCMA, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Mouse BAFF, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA.
Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind Mouse BAFF, mFc Tag with an affinity constant of 39.20 nM as determined in BLI assay (Gator Prime).
Loaded Mouse BCMA, His Tag on Anti-His-Biosensor can bind Mouse APRIL, hFc Tag with an affinity constant of 7.09 nM as determined in BLI assay (Gator Prime).
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