Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Asp25-Ser262
Target:
B7-H6/NCR3LG1
Human B7-H6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human B7-H6 is greater than 95% as determined by SEC-HPLC.
Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Human NKp30, His Tag with an affinity constant of 0.292 uM as determined in SPR assay (Biacore T200).
Immobilized Human NKp30, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test).
Loaded Human B7-H6, hFc Tag on ProA-Biosensor, can bind Human NKp30, His Tag with an affinity constant of 0.51 µM as determined in BLI assay (Gator).
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