Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Form:
Lyophilized
Sequence:
Trp21-Asn343
Target:
CD155/PVR
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 uM as determined in SPR assay (Biacore T200).
Human CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human CD96, mFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA.
Immobilized Human TIGIT, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.38ug/ml determined by ELISA.
The purity of Human CD155 is greater than 90% as determined by SEC-HPLC.
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