Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with His tag at the C-Terminus of CD3D. It contains Asp23-Asp126 (CD3E) and Phe22-Ala105 (CD3D).
Molecular Weight:
The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 28-38 kDa and 25 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
Target:
CD3E&CD3D/CD3 epsilon&CD3 delta
The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
Human CD3E&CD3D, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).
Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 39.3ng/ml determined by ELISA (QC Test).
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