Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus of CD3E and CD3G.It contains Asp23-Asp126 (CD3E) and Gln23-Ser116 (CD3G).
Molecular Weight:
The protein has a predicted MW of 37.8 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Asp23-Asp126 (CD3E) & Gln23-Ser116 (CD3G)
Target:
CD3E&CD3G/CD3 epsilon&CD3 gamma
The purity of Human CD3E&CD3G/CD3 epsilon&CD3 gamma was greater than 95% as determined by SEC-HPLC.
Human CD3E&CD3G/CD3 epsilon&CD3 gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Tebentafusp, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD3E&CD3G, hFc Tag with the EC50 of 0.22 ug/ml determined by ELISA.
Immobilized Human CD3E&CD3G, hFc Tag at 1 ug/ml (100 ul/Well). Dose response curve for Anti-CD3E&CD3G Ab.2, mFc Tag with the EC50 of 20.7 ng/ml determined by ELISA (QC Test).
Immobilized Human CD3E&CD3G, hFc Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3G Ab.1, hFc Tag with the EC50 of 1.22 ug/ml determined by ELISA.
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