Human CD73/NT5E Protein (CD7-HM173)

Catalog Number: KAB-CD7-HM173
Article Name: Human CD73/NT5E Protein (CD7-HM173)
Biozol Catalog Number: KAB-CD7-HM173
Supplier Catalog Number: CD7-HM173
Alternative Catalog Number: KAB-CD7-HM173-100UG, KAB-CD7-HM173-500UG
Manufacturer: KactusBio
Category: Proteine/Peptide
Species Reactivity: Human
Alternative Names: CD73, NT5E, NTE, 5 nucleotidase (CD73), 5-NT, 5-NT, E5NT, EC 3.1.3.5, eN, eNT, NT, NT55-nucleotidase, NT5, NTE, CALJA
Recombinant Human CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp27-Lys547.
Molecular Weight: The protein has a predicted MW of 58.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Tag: C-His
UniProt: P21589
Buffer: Lyophilized from 0.22µm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Expression System: HEK293
Purity: > 90% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form: Lyophilized
Sequence: Trp27-Lys547
Target: CD73/NT5E
The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.
Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
Loaded Anti-CD73 Antibody, hFc Tag on ProA-Biosensor can bind Human CD73, His Tag with an affinity constant of 0.31 nM as determined in BLI assay (Gator Prime).