Lyophilized from 0.22µm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 90% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Trp27-Lys547
Target:
CD73/NT5E
The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.
Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
Loaded Anti-CD73 Antibody, hFc Tag on ProA-Biosensor can bind Human CD73, His Tag with an affinity constant of 0.31 nM as determined in BLI assay (Gator Prime).
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