Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose / 15% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gln19-Lys140
Target:
CD47
Mouse CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD47 is greater than 95% as determined by SEC-HPLC.
Mouse CD47, hFc Tag captured on CM5 Chip via Protein A can bind Mouse SIRP alpha, His Tag with an affinity constant of 28.33 nM as determined in SPR assay (Biacore T200).
Immobilized Mouse SIRP alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 38.6ng/ml determined by ELISA (QC Test).
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