Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM416B)
Biozol Catalog Number:
KAB-CDA-HM416B
Supplier Catalog Number:
CDA-HM416B
Alternative Catalog Number:
KAB-CDA-HM416B-100UG, KAB-CDA-HM416B-500UG
Manufacturer:
KactusBio
Category:
Proteine/Peptide
Species Reactivity:
Human
Conjugation:
Biotin
Alternative Names:
Fc gamma RIIIA, Fc gamma RIIIA (V176), FCGR3, FCGR3A, FCGRIII, FcgRIIIA, FcR-10, CD16A, FCG3, IGFR3, FcRIIIa, FcRIII, CD16, IMD20, FcgammaRIIIA, Fc gammaRIIIA, Fcgamma RIIIA, Fc gamma RIIIA
Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly17-Gln208(F176).
Molecular Weight:
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gly17-Gln208(F176)
Target:
Fc gamma RIIIA/CD16a
Biotinylated Human Fc gamma RIIIA (F176) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Fc gamma RIIIA (F176), His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14uM as determined in SPR assay (Biacore T200).
Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176), His-Avi Tag with the EC50 of 18.9ng/ml determined by ELISA (QC Test).
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