Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein (CDA-HM432B)
Biozol Catalog Number:
KAB-CDA-HM432B
Supplier Catalog Number:
CDA-HM432B
Alternative Catalog Number:
KAB-CDA-HM432B-100UG, KAB-CDA-HM432B-500UG
Manufacturer:
KactusBio
Category:
Proteine/Peptide
Species Reactivity:
Human
Conjugation:
Biotin
Alternative Names:
Fc gamma RIIA, FCG2, FCGR2, FCGR2A, FCGR2A1, FcgRIIA, FCRIIA, fcRII-a, FCG2, CD32A, FcgammaRIIA, Fc gammaRIIA, Fcgamma RIIA, Fc gamma RIIA
Recombinant Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln34-Ile218(H167).
Molecular Weight:
The protein has a predicted MW of 23.4 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gln34-Ile218(H167)
Target:
Fc gamma RIIA/CD32a
Human Fc gamma RIIA (H167) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Fc gamma RIIA (H167) is greater than 95% as determined by SEC-HPLC.
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.680 uM as determined in SPR assay (Biacore T200).
Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 1.44 uM as determined in SPR assay (Biacore T200).
Loaded Biotinylated Human Fc gamma RIIA (H167), His-Avi Tag on Anti-His-Biosensor can bind Rituximab, hFc Tag with an affinity constant of 3.29 µM as determined in BLI assay (Gator Prime).
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