Recombinant Human CDCP1 ATF&CTF Protein is expressed from HEK293 with His tag at the C-terminus.It contains Phe30-Arg368 and Lys369-Thr667 fused by polypeptide linker.
Molecular Weight:
The protein has a predicted MW of 74.70 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Phe30-Arg368 & Lys369-Thr667
Target:
CDCP1
The purity of Human CDCP1 ATF&CTF is greater than 95% as determined by SEC-HPLC.
Human CDCP1 ATF&CTF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human CDCP1 ATF&CTF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Human CDCP1 ATF&CTF, His Tag with an affinity constant of 19.90 nM as determined in BLI assay (Gator Prime).
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