Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Glu33-Asn190
Target:
M-CSF/CSF-1
Human M-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.
Human M-CSF, No Tag immobilized on CM5 Chip can bind Human M-CSF R, hFc Tag with an affinity constant of 2.06 nM as determined in SPR assay (Biacore T200).
Immobilized Human M-CSF, No Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 13.5ng/ml determined by ELISA.
Measured in a cell proliferation assay using RAW264.7 cells.The ED50-for this effect is 1-10 ng/mL.
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