Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Glu33-Asn190
Target:
M-CSF/CSF-1
Human M-CSF on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.
Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Measured in a cell proliferation assay using RAW264.7 cells.The ED50 for this effect is 2.5 - 7.0 ng/mL.
Immobilized Human M-CSF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test).
Loaded Human M-CSF, His Tag on Anti-His-Biosensor can bind Human M-CSF R, No Tag with an affinity constant of 0.46 µM as determined in BLI assay (Gator Prime).
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