Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Lys36-Asp161
Target:
CTLA-4/CD152
Human CTLA-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CTLA-4 is greater than 95% as determined by SEC-HPLC.
Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA-4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
Immobilized Human B7-2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
Immobilized Human B7-1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
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