Lyophilized from 0.22 µm filtered solution in PBS, 200 mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Form:
Lyophilized
Sequence:
Ala27-Arg490
Target:
DLL3
Human DLL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Anti-DLL3 Antibody captured on CM5 Chip via Protein A can bind Human DLL3, His Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Immobilized Human DLL3, His Tag at 0.1 ug/mL (100 ul/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA (QC Test).
Loaded Anti-DLL3 Antibody, hFc Tag on ProA-Biosensor can bind Human DLL3, His Tag with an affinity constant of 0.35 nM as determined in BLI assay (Gator Prime).
The purity of Human DLL3 is greater than 90% as determined by SEC-HPLC.
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