Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE.,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Ser27-Pro524
Target:
DLL4
Human DLL4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human DLL4 is greater than 95% as determined by SEC-HPLC.
Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 uM as determined in SPR assay (Biacore T200).
Immobilized Human Notch 1, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 10.3 ng/ml determined by ELISA (QC Test).
Immobilized Human DLL4, hFc Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 23.5 ng/ml determined by ELISA.
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