Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Arg152-Glu378
Target:
FGFR2 beta (IIIb)
Human FGFR2 beta (IIIb) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC.
Anti-FGFR2 Antibody, hFc Tag can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 1.98nM as determined in a SPR assay (Biacore T200).
Serial dilutions of Anti-FGFR2 Antibody were added into Human FGFR2 beta (lllb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 1.74ng/ml.
Immobilized Human FGFR2 beta (IIIb), His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR2 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
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