Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Leu72-Leu251
Target:
TNFSF15
Cynomolgus/Rhesus macaque TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 95% as determined by SEC-HPLC.
Mouse DR3, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque TNFSF15, His Tag with an affinity constant of 11.70 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
Immobilized Cynomolgus/Rhesus macaque TNFSF15, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 20.8ng/ml determined by ELISA.
The purity of Cynomolgus/Rhesus macaque TNFSF15 is greater than 85% and the molecular weight of this protein is around 60-98 kDa as determined by SEC-MALS.
Loaded Human DR3, hFc Tag on ProA-Biosensor, can bind Cynomolgus/Rhesus macaque TNFSF15, His Tag with an affinity constant of 1.30 nM as determined in BLI assay (Gator).
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