Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Asp91-Leu251
Target:
TNFSF15
Human TNFSF15 Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15 (Trimer) , His Tag with an affinity constant of 3.06 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFSF15 Trimer, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA.
Human DR3, hFc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 2.2ug/ml determined by ELISA.
Immobilized Human TNFSF15 (Trimer), His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 38.5ng/ml determined by ELISA.
The purity of Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.
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