Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield. NOTE: A PH 7.0 is ideal for optimal performance and the cofactor Mg2+ is required for enzymatic activity. This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF 038030.
Buffer:
Liquid
Figure 1. The addition of inorganic pyrophosphatase to IVT reactions can effectively increase mRNA yield. Lane 1 was without inorganic pyrophosphatase, while lane 2 had added inorganic pyrophosphatase.
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