Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Form:
Lyophilized
Sequence:
Leu20-Pro365
Target:
IL-6 R alpha/CD126
Human IL-6 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA (QC Test).
Immobilized Human IL-6 R alpha, hFc Tag at 2ug/ml (100ul/well) on the Human IL-6, No Tag precoated plate (5ug/ml). Dose response curve for Human gp130, His Tag with the EC50 of 49.6ng/ml determined by ELISA.
Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-6, No Tag with an affinity constant of 16.50 nM as determined in BLI assay (Gator Prime).
The purity of Human IL-6 R alpha is greater than 90% as determined by SEC-HPLC.
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