Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Leu20-Pro365
Target:
IL-6 R alpha/CD126
Human IL-6 R alpha on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of- Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC.
Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-6, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test).
Loaded Human IL-6 R alpha, His Tag on Anti-His-Biosensor can bind Human IL-6, No Tag with an affinity constant of 30.80 nM as determined in BLI assay (Gator Prime).
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