Recombinant Rhesus macaque LILRB1/CD85j/ILT2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Met1-His474.
Molecular Weight:
The protein has a predicted MW of 75.2 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Bis-Tris PAGE result.
Tag:
C-hFc (IgG1)
UniProt:
NP_001035762.2
Buffer:
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Met1-His474
Target:
LILRB1/CD85j/ILT2
Rhesus macaque LILRB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque LILRB1 is greater than 95% as determined by SEC-HPLC.
Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200).
Anti-LILRB1 Antibody immobilized on CM5 Chip can bind Rhesus macaque LILRB1, hFc Tag with an affinity constant of 47.69 nM as determined in SPR assay (Biacore T200).
Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test).
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