Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein (MHC-HM402T)
Biozol Catalog Number:
KAB-MHC-HM402T
Supplier Catalog Number:
MHC-HM402T
Alternative Catalog Number:
KAB-MHC-HM402T-100UG, KAB-MHC-HM402T-500UG
Manufacturer:
KactusBio
Category:
Proteine/Peptide
Species Reactivity:
Human
Alternative Names:
HLA0201, MHC I, gp100, Glycoprotein 100, ME20-M, P100
Recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide.
Molecular Weight:
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide
Target:
HLA-A*02:01&B2M&GP100 (YLEPGPVTA)
Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer was greater than 95% as determined by SEC-HPLC.
Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag immobilized on CM5 Chip can bind Tebentafusp with an affinity constant of 0.196 nM as determined in SPR assay (Biacore T200).
Immobilized Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
Immobilized Tebentafusp, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag with the EC50 of 39.5ng/ml determined by ELISA.
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