Recombinant Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide.
Molecular Weight:
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide
Target:
HLA-A*02:01&B2M&AFP (FMNKFIYEI)
Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer is greater than 95% as determined by SEC-HPLC.
Immobilized HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR at 2ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag with the EC50 of 26.6ng/ml determined by ELISA.
Immobilized Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test).
Human HLA-A*02:01&B2M&AFP (FMNKFIEl) Tetramer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP (FMNKFIYEI) TCR with an affinity constant of 0.111 uM as determined in SPR assay (Biacore T200).
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