PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (MHC-HM43RTP)

Catalog Number: KAB-MHC-HM43RTP
Article Name: PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (MHC-HM43RTP)
Biozol Catalog Number: KAB-MHC-HM43RTP
Supplier Catalog Number: MHC-HM43RTP
Alternative Catalog Number: KAB-MHC-HM43RTP-100UG, KAB-MHC-HM43RTP-500UG
Manufacturer: KactusBio
Category: Proteine/Peptide
Species Reactivity: Human
Alternative Names: MHC, HLA-A*02:01, Peptide Ready
Recombinant PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer is assembled by biotinylated monomer and PE-Labeled streptavidinIt contains Gly25-Thr305 (HLA-A*02:01) and Ile21-Met119 (B2M).
Tag: C-His-Avi
UniProt: A0A140T913
Buffer: Supplied as 0.22 µm filtered solution in PBS, 100mM L-arginine, 0.2% BSA (pH 7.4).
Expression System: HEK293
Form: Liquid
Sequence: Gly25-Thr305 (HLA-A*02:01) & Ile21-Met119 (B2M)
Target: Peptide Ready HLA-A*02:01&B2M
Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA.
1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (Cat. MHC-HM43RTP) (Loaded PRAME Peptide SLLQHLIGL) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal.