PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (MHC-HM443TP)

Catalog Number: KAB-MHC-HM443TP
Article Name: PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (MHC-HM443TP)
Biozol Catalog Number: KAB-MHC-HM443TP
Supplier Catalog Number: MHC-HM443TP
Alternative Catalog Number: KAB-MHC-HM443TP-100UG, KAB-MHC-HM443TP-500UG
Manufacturer: KactusBio
Category: Proteine/Peptide
Species Reactivity: Human
Alternative Names: PRAME, OIP-4, OIP4, MAPE,
Recombinant PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide.
Tag: C-His-Avi
UniProt: A0A140T913
Buffer: Supplied as 0.22 µm filtered solution in PBS, 0.2% BSA (pH 7.4).
Expression System: HEK293
Form: Liquid
Sequence: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide
Target: HLA-A*02:01&B2M&PRAME (SLLQHLIGL)
Immobilized PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA.
1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer (Cat. No.MHC-HM443TP) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal.