Recombinant Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein is expressed from E.coli with with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVVGADGVGK peptide.
Molecular Weight:
The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
E.coli
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVVGADGVGK peptide
Target:
HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK)
HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer is greater than 95% as determined by SEC-HPLC.
Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag captured on CM5 Chip via anti-his antibody can bind HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR with an affinity constant of 6.93 nM as determined in SPR assay (Biacore T200).
Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 5ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 66.0ng/ml determined by ELISA (QC Test).
Immobilized Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
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