Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Glu24-Gln308
Target:
MICA
The purity of Human MICA is greater than 95% as determined by SEC-HPLC.
Human MICA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Human MICA, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 0.36 uM as determined in SPR assay (Biacore T200).
Immobilized Human MICA, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MICA Antibody, hFc Tag with the EC50 of 0.45ug/ml determined by ELISA.
Immobilized Human NKG2D, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
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