Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Ala23-Gly298
Target:
MICB
Human MICB on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human MICB is greater than 95% as determined by SEC-HPLC.
Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Human MICB, His Tag with an affinity constant of 83.31 nM as determined in SPR assay (Biacore T200).
Immobilized Human MICB, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICB Antibody, hFc Tag with the EC50 of 42.4ng/ml determined by ELISA.
Immobilized Human MICB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.29ug/ml determined by ELISA (QC Test).
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