Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
Form:
Lyophilized
Sequence:
Ala23-Gly298
Target:
MICB
Biotinylated Human MICB on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human MICB, His Tag with an affinity constant of 60.38 nM as determined in SPR assay (Biacore T200).
Immobilized Anti-MICB Antibody, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human MICB, His Tag with the EC50 of 0.43ug/ml determined by ELISA.
Immobilized Biotinylated Human MICB, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA (QC Test).
The purity of Biotinylated Human MICB is greater than 90% as determined by SEC-HPLC.
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