Biotinylated Human MSLN/Mesothelin Protein (MSL-HM580B)
Biozol Catalog Number:
KAB-MSL-HM580B
Supplier Catalog Number:
MSL-HM580B
Alternative Catalog Number:
KAB-MSL-HM580B-100UG, KAB-MSL-HM580B-500UG
Manufacturer:
KactusBio
Category:
Proteine/Peptide
Species Reactivity:
Human
Conjugation:
Biotin
Alternative Names:
MSLN, CAK1, Mesothelin, MPF, MPFSMRP, SMR
Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Glu296-Gly580.
Molecular Weight:
The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Glu296-Gly580
Target:
MSLN/Mesothelin
Biotinylated Human MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human MSLN is greater than 95% as determined by SEC-HPLC.
Use Biotinylated Human MSLN-hFc-Avi protein to detect the expression rate of Anti-MSLN-CAR positive cell. 293T cells and anti-MSLN CAR-293T cells were incubated with Biotinylated human MSLN-hFc-Avi Tag. Non-transfected 293T cells and Fc-labeled protein control were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human MSLN.
Immobilized Anti-MSLN Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA.
Immobilized Human CA125, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 44.1ng/ml determined by ELISA (QC Test).
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