Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gln32-Leu360
Target:
Nectin-2/CD112
Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200).
Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test).
Human PVRIG, mFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 87.49 nM as determined in SPR assay (Biacore T200).
The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC.
Human Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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