Recombinant Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu19-Thr138.
Molecular Weight:
The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result.
Tag:
C-hFc (IgG1)
UniProt:
AAH52582
Buffer:
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Leu19-Thr138
Target:
NKp30/NCR3/CD337
Human NKp30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human NKp30 is greater than 95% as determined by SEC-HPLC.
Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H6, His Tag (Cat. BH7-HM176) with an affinity constant of 48.67 nM as determined in SPR assay (Biacore T200).
Immobilized Human NKp30, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA (QC Test).
Loaded Human NKp30, hFc Tag on Anti-His-Biosensor can bind Human B7-H6, His Tag (Cat. BH7-HM176) with an affinity constant of 0.56 µM as determined in BLI assay (Gator Prime).
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