Recombinant Biotinylated Human NPR1/NPRA Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly33-Glu473.
Molecular Weight:
The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
Tag:
C-His-Avi
UniProt:
NP_000897.3
Buffer:
Supplied as 0.22 µm filtered solution in PBS (pH 7.4).
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Liquid
Sequence:
Gly33-Glu473
Target:
NPR1/NPRA
The purity of Biotinylated Human NPR1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human NPR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Biotinylated Human NPR1, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
Anti-NPR1 Ab.2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NPR1, His Tag with an affinity constant of 2.34 µM as determined in SPR assay (Biacore T200).
Loaded Anti-NPR1 Ab., hFc Tag on ProA-Biosensor can bind Biotinylated NPR1, His-Avi Tag with an affinity constant of 3.80 nM as determined in BLI assay (Gator Prime).
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