Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Leu25-Gln167
Target:
PD-1/PDCD1
Cynomolgus PD-1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Cynomolgus PD-1 is greater than 95% as determined by SEC-HPLC.
Cynomolgus PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus PD-L1, His Tag with an affinity constant of 1.28 uM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus PD-1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 25.0ng/ml determined by ELISA (QC Test).
Loaded Cynomolgus PD-1, hFc Tag on ProA-Biosensor, can bind Cynomolgus PD-L2, His Tag with an affinity constant of 0.28 µM as determined in BLI assay (Gator).
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