Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Leu25-Gln167
Target:
PD-1/PDCD1
The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC.
Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 uM as determined in SPR assay (Biacore T200).
Biotinylated Human PD-1 on Bis-Tris PAGE under reduced. The purity is greater than 95%.
Immobilized Biotinylated Human PD-1, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test).
Loaded Biotinylated Human PD-1, His Tag on Streptavidin-Biosensor can bind Human PD-L1, His Tag with an affinity constant of 0.20 µM as determined in BLI assay (Gator Prime).
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