Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Phe19-Arg238
Target:
PD-L1/B7-H1
The purity of Biotinylated Human PD-L1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Anti-PD-L1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human PD-L1, His Tag with the EC50 of 9.4ng/ml determined by ELISA.
Immobilized Biotinylated Human PD-L1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human PD-1, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
Loaded Biotinylated Human PD-L1, His Tag on Streptavidin-Biosensor can bind Human PD-1, His Tag with an affinity constant of 0.94 µM as determined in BLI assay (Gator Prime).
Serial dilutions of Anti-PD-1 Antibody were added into Human PD-1, hFc Tag: Biotinylated Human PD-L1, His Avi Tag binding reactions. The half maximal inhibitiory concentration (IC50) is 0.22ug/ml.
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