Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Gly63-Asp244
Target:
RANKL/TNFSF11/CD254
Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Human RANKL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human RANKL, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6 ng/ml determined by ELISA (QC Test).
The purity of Human RANKL is greater than 95% as determined by SEC-HPLC.
Immobilized Human RANKL, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFRSF11B, His Tag with the EC50 of 16.2ng/ml determined by ELISA.
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