RNase III is a double-stranded RNA (dsRNA) -specific endoribonuclease that can cleave dsRNA into 18-25 bp interfering RNAs (siRNA) with 2 nucleotide 3 overhangs, 5 phosphate and 3 hydroxyl. RNase III can be used to generate siRNA library from long dsRNA for gene silencing and target validation, RNase III can also be used to remove dsRNA, a side product with immunogenicity, that is generated during in vitro transcription.
Buffer:
Liquid
RNase III on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Digestion of 1µg 500bp dsRNA with RNase III, followed by Agarose gel analysis.
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