Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Glu31-Arg370
Target:
SIRP alpha/CD172a
Serial dilutions of Anti-SIRP alpha Antibody were added into Human SIRP alpha, His Tag: Biotinylated Human CD47, His Tag binding reactions.The half maximal inhibitory concentration (IC50) is 73.46ng/ml.
The purity of Human SIRP alpha is greater than 95% as determined by SEC-HPLC.
Human SIRP alpha, His Tag captured on Protein A Chip via Anti-His Antibody can bind Human CD47, hFc Tag with an affinity constant of 20.6nM as determined in SPR assay (Biacore T200).
Anti-SIRP alpha Antibody captured on CM5 Chip via Protein A can bind Human Sirpa, His Tag with an affinity constant of 2.81 nM as determined in SPR assay (Biacore T200).
Human SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human SIRP alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
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