Recombinant Human SIRP alpha V2/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg369.
Molecular Weight:
The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Tag:
C-His
UniProt:
AAH26692.1
Buffer:
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Glu31-Arg369
Target:
SIRP alpha V2/CD172a
Human SIRP alpha V2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SIRP alpha V2 is greater than 95% as determined by SEC-HPLC.
Serial dilutions of Anti-SIRP alpha Antibody, hFc Tag were added into Human SIRP alpha V2, His Tag : Biotinylated Human CD47 Protein, His Tag binding reactioins.The half maximal inhibitiory concentration (IC50) is 54.14ng/ml.
Human SIRP alpha V2, His Tag captured on Protein A Chip via Anti-His Antibody can bind Anti-SIRP alpha Ab., hFc Tag with an affinity constant of 9.11nM as determined in SPR assay (Biacore T200).
Immobilized Human SIRP alpha V2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
Immobilized Human SIRP alpha V2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA (QC Test).
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