Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Ser33-Glu251
Target:
Coagulation factor III/Tissue Factor
The purity of Human Coagulation factor III is greater than 95% as determined by SEC-HPLC.
Human Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human Coagulation factor III, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation factor III Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test).
Anti-Coagulation factor III Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human Coaqulation Factor lll, His Tag with an affinity constant of 9.25 nM as determined in SPR assay (Biacore T200).
Loaded Anti-Coagulation factor III Antibody, hFc Tag on Protein A-Biosensor, can bind Human Coaqulation Factor lll, His Tag with an affinity constant of 5.57 nM as determined in BLI assay (Gator).
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