Biotinylated Human Transferrin R/CD71 Protein (TFR-HM401B)
Biozol Catalog Number:
KAB-TFR-HM401B
Supplier Catalog Number:
TFR-HM401B
Alternative Catalog Number:
KAB-TFR-HM401B-100UG, KAB-TFR-HM401B-500UG
Manufacturer:
KactusBio
Category:
Proteine/Peptide
Species Reactivity:
Human
Conjugation:
Biotin
Alternative Names:
TR, TfR1, CD71, sTfR, T9, p90, TFRC, TFR
Recombinant Biotinylated Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.
Molecular Weight:
The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS, 200mM L-arginine, 8% trehalose (pH 7.4).
Expression System:
HEK293
Purity:
> 90% as determined by Bis-Tris PAGE
Form:
Lyophilized
Sequence:
Cys89-Phe760
Target:
Transferrin R/CD71
Biotinylated Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Biotinylated Human Transferrin R, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test).
Human Transferrin, His Tag immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 7.73 nM as determined in SPR assay (Biacore T200).
Loaded Anti-Transferrin R Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 0.24 nM as determined in BLI assay (Gator Prime).
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