Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form:
Lyophilized
Sequence:
Tyr29-Gln159 (R127A, R130A)
Target:
TSLP
Immobilized Human TSLP (R127A, R130A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
The purity of Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.
Immobilized Human TSLP (R127A, R130A), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Human TSLP (R127A, R130A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Human TSLP (R127A, R130A), His Tag with an affinity constant of 12.70 nM as determined in BLI assay (Gator Prime).
Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Human TSLP (R127A, R130A), His Tag with an affinity constant of 0.41 nM as determined in BLI assay (Gator Prime).
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