Human VEGF165 Protein (VEG-HM065)

Catalog Number: KAB-VEG-HM065
Article Name: Human VEGF165 Protein (VEG-HM065)
Biozol Catalog Number: KAB-VEG-HM065
Supplier Catalog Number: VEG-HM065
Alternative Catalog Number: KAB-VEG-HM065-100UG, KAB-VEG-HM065-500UG
Manufacturer: KactusBio
Category: Proteine/Peptide
Species Reactivity: Human
Alternative Names: VEGF, VEGFA, MVCD1, VAS, VEGFMGC70609, VPF, RP1-261G23.1, MGC70609,VEGF-165
Recombinant Human VEGF165 Protein is expressed from HEK293 without tag.It contains Ala27-Arg191.
Molecular Weight: The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Bis-Tris PAGE result.
Tag: No Tag
UniProt: P15692
Buffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System: HEK293
Purity: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Form: Lyophilized
Sequence: Ala27-Arg191
Target: VEGF165
Human VEGF165 on Bis-Tris PAGE under reduced condition and Non reduced (N) condition. The purity is greater than 95%.
Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R2, HisTag (Cat. VGF-HM1R2) with the EC50 of 0.35ug/ml determined by ELISA.
Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 46.4ng/ml determined by ELISA.
Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50-for this effect is < 10 ng/mL (QC Test).
Immobilized Human VEGF165, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
Serial dilutions of Anti-VEGF165 Antibody were added into Human VEGF165, No Tag: Biotinylated Human VEGF R2, His Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.30ug/ml.
The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC.