Recombinant Human Wnt Surrogate-Fc Fusion Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.
Molecular Weight:
The protein has a predicted MW of 58.50 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
Tag:
C-hFc (IgG1)
Buffer:
Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Purity:
> 95% as determined by Bis-Tris PAGE
Form:
Lyophilized
Target:
Wnt Surrogate
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50-for this effect is 2-12 ng/mL (QC Test).
Human Wnt Surrogate-Fc Fusion on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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