Homologous to the poliovirus receptor (PVR/CD155), the nectin immunoglobulin superfamily comprises four known isoforms (-1, -2, -3, and -4). The ectodomain of nectin family members comprises three Ig-like domains (V, C, C). Nectins localize at the adherens junctions (AJ) in epithelial and endothelial cells where they serve as adhesion molecules. Actin-based AJs play a role in mechanical adhesion, cellular morphogenesis and cellular differentiation. Nectin associates with the Actin cytoskeleton through its interaction with the Actin filament-binding protein afadin. Nectin 4 and afadin co-localize at cadherin-based adherens junctions in MDCKII epithelial cells. Nectin 4 and Nectin 3 share a common binding region in the V domain of Nectin 1 and thus compete for Nectin 1 binding. The Nectin 3/4 binding domain maps to the C-C-C-D b strands of the V domain of Nectin 1. Unlike other nectins, which are more widely expressed, Nectin 4 is mainly expressed in the placenta.
200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
SDS-PAGE Analysis of Purified NECTIN4 Recombinant Rabbit Monoclonal Antibody (NECTIN4/13438R). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human placenta stained with Nectin 4 Recombinant Rabbit Monoclonal Antibody (NECTIN4/13438R). Inset: PBS instead of primary antibody, secondary only negative control.
Flow cytometric analysis of cell-surface Nectin-4 on T47D (human ductal breast epithelial tumor epithelial) cells. T47D cells were stained with recombinant rabbit anti,AiNectin-4 monoclonal antibody (NECTIN4/13438R) at dilutions of 1:50 (4 g/ml, dark red), 1:100 (2 g/ml, orange), 1:200 (1 g/ml, light green) and 1:400 (0.5 g/ml, dark green), then incubated with goat anti-rabbit IgG,AiCF488. CF488 fluorescence intensity (log,CA,CA scale, x-axis) is plotted against relative cell count (y-axis). Progressive rightward shifts with increasing antibody concentration demonstrate dilution-dependent binding of Nectin-4. The blue histogram corresponds to the isotype control.
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